Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus.
Identifieur interne : 002123 ( Main/Exploration ); précédent : 002122; suivant : 002124Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus.
Auteurs : Jessy L. Labbé [États-Unis] ; David J. Weston [États-Unis] ; Nora Dunkirk [États-Unis] ; Dale A. Pelletier [États-Unis] ; Gerald A. Tuskan [États-Unis]Source :
- Frontiers in plant science [ 1664-462X ] ; 2014.
Abstract
Mycorrhiza helper bacteria (MHB) are known to increase host root colonization by mycorrhizal fungi but the molecular mechanisms and potential tripartite interactions are poorly understood. Through an effort to study Populus microbiome, we isolated 21 Pseudomonas strains from native Populus deltoides roots. These bacterial isolates were characterized and screened for MHB effectiveness on the Populus-Laccaria system. Two additional Pseudomonas strains (i.e., Pf-5 and BBc6R8) from existing collections were included for comparative purposes. We analyzed the effect of co-cultivation of these 23 individual Pseudomonas strains on Laccaria bicolor "S238N" growth rate, mycelial architecture and transcriptional changes. Nineteen of the 23 Pseudomonas strains tested had positive effects on L. bicolor S238N growth, as well as on mycelial architecture, with strains GM41 and GM18 having the most significant effect. Four of seven L. bicolor reporter genes, Tra1, Tectonin2, Gcn5, and Cipc1, thought to be regulated during the interaction with MHB strain BBc6R8, were induced or repressed, while interacting with Pseudomonas strains GM17, GM33, GM41, GM48, Pf-5, and BBc6R8. Strain GM41 promoted the highest roots colonization across three Populus species but most notably in P. deltoides, which is otherwise poorly colonized by L. bicolor. Here we report novel MHB strains isolated from native Populus that improve L. bicolor root colonization on Populus. This tripartite relationship could be exploited for Populus species/genotypes nursery production as a means of improving establishment and survival in marginal lands.
DOI: 10.3389/fpls.2014.00579
PubMed: 25386184
PubMed Central: PMC4208408
Affiliations:
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Labbé</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Weston, David J" sort="Weston, David J" uniqKey="Weston D" first="David J" last="Weston">David J. Weston</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Dunkirk, Nora" sort="Dunkirk, Nora" uniqKey="Dunkirk N" first="Nora" last="Dunkirk">Nora Dunkirk</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Pelletier, Dale A" sort="Pelletier, Dale A" uniqKey="Pelletier D" first="Dale A" last="Pelletier">Dale A. Pelletier</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mycorrhiza helper bacteria (MHB) are known to increase host root colonization by mycorrhizal fungi but the molecular mechanisms and potential tripartite interactions are poorly understood. Through an effort to study Populus microbiome, we isolated 21 Pseudomonas strains from native Populus deltoides roots. These bacterial isolates were characterized and screened for MHB effectiveness on the Populus-Laccaria system. Two additional Pseudomonas strains (i.e., Pf-5 and BBc6R8) from existing collections were included for comparative purposes. We analyzed the effect of co-cultivation of these 23 individual Pseudomonas strains on Laccaria bicolor "S238N" growth rate, mycelial architecture and transcriptional changes. Nineteen of the 23 Pseudomonas strains tested had positive effects on L. bicolor S238N growth, as well as on mycelial architecture, with strains GM41 and GM18 having the most significant effect. Four of seven L. bicolor reporter genes, Tra1, Tectonin2, Gcn5, and Cipc1, thought to be regulated during the interaction with MHB strain BBc6R8, were induced or repressed, while interacting with Pseudomonas strains GM17, GM33, GM41, GM48, Pf-5, and BBc6R8. Strain GM41 promoted the highest roots colonization across three Populus species but most notably in P. deltoides, which is otherwise poorly colonized by L. bicolor. Here we report novel MHB strains isolated from native Populus that improve L. bicolor root colonization on Populus. This tripartite relationship could be exploited for Populus species/genotypes nursery production as a means of improving establishment and survival in marginal lands. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">25386184</PMID><DateCompleted><Year>2014</Year><Month>11</Month><Day>11</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>5</Volume><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus.</ArticleTitle><Pagination><MedlinePgn>579</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2014.00579</ELocationID><Abstract><AbstractText>Mycorrhiza helper bacteria (MHB) are known to increase host root colonization by mycorrhizal fungi but the molecular mechanisms and potential tripartite interactions are poorly understood. Through an effort to study Populus microbiome, we isolated 21 Pseudomonas strains from native Populus deltoides roots. These bacterial isolates were characterized and screened for MHB effectiveness on the Populus-Laccaria system. Two additional Pseudomonas strains (i.e., Pf-5 and BBc6R8) from existing collections were included for comparative purposes. We analyzed the effect of co-cultivation of these 23 individual Pseudomonas strains on Laccaria bicolor "S238N" growth rate, mycelial architecture and transcriptional changes. Nineteen of the 23 Pseudomonas strains tested had positive effects on L. bicolor S238N growth, as well as on mycelial architecture, with strains GM41 and GM18 having the most significant effect. Four of seven L. bicolor reporter genes, Tra1, Tectonin2, Gcn5, and Cipc1, thought to be regulated during the interaction with MHB strain BBc6R8, were induced or repressed, while interacting with Pseudomonas strains GM17, GM33, GM41, GM48, Pf-5, and BBc6R8. Strain GM41 promoted the highest roots colonization across three Populus species but most notably in P. deltoides, which is otherwise poorly colonized by L. bicolor. Here we report novel MHB strains isolated from native Populus that improve L. bicolor root colonization on Populus. This tripartite relationship could be exploited for Populus species/genotypes nursery production as a means of improving establishment and survival in marginal lands. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Labbé</LastName><ForeName>Jessy L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Weston</LastName><ForeName>David J</ForeName><Initials>DJ</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dunkirk</LastName><ForeName>Nora</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pelletier</LastName><ForeName>Dale A</ForeName><Initials>DA</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tuskan</LastName><ForeName>Gerald A</ForeName><Initials>GA</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>10</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Laccaria bicolor</Keyword><Keyword MajorTopicYN="N">Populus rhizosphere</Keyword><Keyword MajorTopicYN="N">fungal-bacterial-Populus interactions</Keyword><Keyword MajorTopicYN="N">mutualistic interactions</Keyword><Keyword MajorTopicYN="N">mycorrhiza helper bacteria</Keyword><Keyword MajorTopicYN="N">mycorrhizal fungi</Keyword><Keyword MajorTopicYN="N">soil microbiology</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>08</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">16159334</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2007;176(1):22-36</Citation><ArticleIdList><ArticleId IdType="pubmed">17803639</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2006 Jan;8(1):1-10</Citation><ArticleIdList><ArticleId IdType="pubmed">16343316</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2005 Jul;23(7):873-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15980861</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Ecol. 2002 Mar 1;39(3):219-27</Citation><ArticleIdList><ArticleId IdType="pubmed">19709201</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2007 Feb;97(2):233-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18944380</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1991 Jan;173(2):697-703</Citation><ArticleIdList><ArticleId IdType="pubmed">1987160</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1997 Jan;63(1):139-44</Citation><ArticleIdList><ArticleId IdType="pubmed">16535478</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2014 Jan 21;86(2):1083-90</Citation><ArticleIdList><ArticleId IdType="pubmed">24377265</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2011 Sep;77(17):5934-44</Citation><ArticleIdList><ArticleId IdType="pubmed">21764952</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 2007 Aug;52(2):77-85</Citation><ArticleIdList><ArticleId IdType="pubmed">17632722</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2007;175(4):743-55</Citation><ArticleIdList><ArticleId IdType="pubmed">17688589</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Ecol. 2002 Jul 1;41(1):37-46</Citation><ArticleIdList><ArticleId IdType="pubmed">19709237</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2005 Mar;15(2):87-91</Citation><ArticleIdList><ArticleId IdType="pubmed">15015061</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Philos Trans R Soc Lond B Biol Sci. 2000 Jun 29;355(1398):815-30; discussion 830-1</Citation><ArticleIdList><ArticleId IdType="pubmed">10905611</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 2012 Nov;194(21):5991-3</Citation><ArticleIdList><ArticleId IdType="pubmed">23045501</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2010 May 13;6(5):e1000943</Citation><ArticleIdList><ArticleId IdType="pubmed">20485560</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1998 Jun;64(6):2304-7</Citation><ArticleIdList><ArticleId IdType="pubmed">9603857</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3148-53</Citation><ArticleIdList><ArticleId IdType="pubmed">14973188</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2007;174(4):892-903</Citation><ArticleIdList><ArticleId IdType="pubmed">17504470</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2009 Feb;19(2):81-90</Citation><ArticleIdList><ArticleId IdType="pubmed">18941805</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2008 Mar 6;452(7183):88-92</Citation><ArticleIdList><ArticleId IdType="pubmed">18322534</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2007;58:435-58</Citation><ArticleIdList><ArticleId IdType="pubmed">17280524</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2010 Jul;5(7):864-7</Citation><ArticleIdList><ArticleId IdType="pubmed">20448463</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2012 Jun;25(6):765-78</Citation><ArticleIdList><ArticleId IdType="pubmed">22375709</ArticleId></ArticleIdList></Reference><Reference><Citation>Syst Appl Microbiol. 2006 Nov;29(7):539-56</Citation><ArticleIdList><ArticleId IdType="pubmed">16919907</ArticleId></ArticleIdList></Reference><Reference><Citation>Antonie Van Leeuwenhoek. 2002 Aug;81(1-4):343-51</Citation><ArticleIdList><ArticleId IdType="pubmed">12448732</ArticleId></ArticleIdList></Reference><Reference><Citation>J Appl Microbiol. 2010 Feb;108(2):703-11</Citation><ArticleIdList><ArticleId IdType="pubmed">19778345</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1999 Nov;65(11):5148-50</Citation><ArticleIdList><ArticleId IdType="pubmed">10543835</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1998 Mar 13;273(11):6565-74</Citation><ArticleIdList><ArticleId IdType="pubmed">9497393</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Labbe, Jessy L" sort="Labbe, Jessy L" uniqKey="Labbe J" first="Jessy L" last="Labbé">Jessy L. 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